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easysep® human whole blood cd3 positive selection kit  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc easysep® human whole blood cd3 positive selection kit
    Impact of whole blood and selected <t>CD3</t> + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%
    Easysep® Human Whole Blood Cd3 Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+cd3++whole+blood+selection+kit/easysep+human+t+cell+isolation+kit/pmc09303291-56-13-21
    Average 90 stars, based on 1 article reviews
    easysep® human whole blood cd3 positive selection kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Utility of assessing CD3 + cell chimerism within the first months after allogeneic hematopoietic stem‐cell transplantation for acute myeloid leukemia"

    Article Title: Utility of assessing CD3 + cell chimerism within the first months after allogeneic hematopoietic stem‐cell transplantation for acute myeloid leukemia

    Journal: Hla

    doi: 10.1111/tan.14557

    Impact of whole blood and selected CD3 + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%
    Figure Legend Snippet: Impact of whole blood and selected CD3 + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%

    Techniques Used:

    Related Articles

    other:

    Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-53BP1 Bethyl Labs Cat# IHC-00001; RRID: AB_513644 Alexa Fluor 488 goat anti-rabbit ThermoFisher Cat# A11034; RRID: AB_2576217 DAPI ThermoFisher Cat# 62248 Rabbit anti-53BP1 Bethyl Labs Cat# A300-272A; RRID: AB_185520 DAPI ThermoFisher Cat# D1306 Mouse monoclonal anti-gH2AX antibody Upstate Cell Signaling Solutions Clone JBW301; RRID: AB_310795 TexasRed-labeled anti-mouse Molecular Probes Invitrogen Cat# T-862; RRID: AB_2556781 Biological Samples PBMCs from buffy coat Oklahoma Blood Institute N/A CD3+ cells from finger prick blood collection Exogen N/A CD3+ cells from total blood draw collection Loma Linda University N/A MCF10A cells ATCC CRL-10317 Chemicals, Peptides, and Recombinant Proteins Ficoll-Paque VWR Cat# 95038-168 Red blood cell lysis buffer VWR Cat# 420301 Triton X-100 Sigma Cat# T8787 Tween 20 Sigma Cat# P1379 Bovine serum albumin Sigma Cat# A9647 Cell-Tak BD Cat# 2173828 Mounting Media Trevigen Cat# 4866-20 Critical Commercial Assays EasySep CD3+ Whole Blood Selection Kit StemCell Technologies Cat# 18081 CellROX Green Flow Cytometry Assay Kit ThermoFisher Cat# C10492 MILLIPLEX MAP Human Cytokine/ Chemokine Magnetic Bead Panel - Immunology Multiplex Assay Millipore Cat# HCYTOMAG-60K Software and Algorithms MATLAB MathWorks https://www.mathworks.com/products/ matlab.html DIPImage Delft University of Technology http://www.diplib.org/download FlowJoTM Software BD https://www.flowjo.com/ Other CellDiscoverer 7 ZEISS SR# 4649000100 CRISP Autofocus System ASI PR# TG-1000-8 Plan-apochromat 40X NA0.95 dry objective Zeiss Cat# N010098 ORCA-Flash4.0 LT+ Digital CMOS camera Hamamatsu Cat# C11440-42U30 FACSCalibur flow cytometer Becton Dickinson Cat# 342973

    Article Title: A one-step assay for sorted CD3 + cell purity and chimerism after hematopoietic stem cell transplantation.
    Article Snippet: A hematopoietic chimerism assay is the laboratory test for monitoring engraftment and quantifying the proportions of donor and recipient cells after hematopoietic stem cell transplantation recipients.. Flow cytometry is the reference method for determining the purity of CD3+ cells on the chimerism of selected CD3+ cells.. In the present study, we developed a single-step procedure that combines the CD3+ purity assay (using the PCR-based Non-T Genomic Detection Kit from Accumol, Calgary, Canada) and the qPCR chimerism monitoring assay (the QTRACE® qPCR assay from Jeta Molecular, Utrecht, the Netherlands).

    Article Title: Utility of assessing CD3 + cell chimerism within the first months after allogeneic hematopoietic stem‐cell transplantation for acute myeloid leukemia
    Article Snippet: CD3 + cells were isolated from 7 ml peripheral blood samples using the EasySep® Human Whole Blood CD3 Positive Selection Kit (STEMCELL Technologies, Vancouver, BC, Canada).

    Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy
    Article Snippet: EasySep CD3+ Whole Blood Selection Kit , StemCell Technologies , Cat# 18081.

    Selection:

    Article Title: Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo
    Article Snippet: .. Briefly, whole PBMCs or T-cell-depleted PBMCs (EasySep CD3 Positive Selection Kit; StemCell Technologies, Vancouver, Canada) were cultured for 13–14 days with 100 U/mL IL-2 (PeproTech, Cranbury, NJ, USA) and 200-μg/mL PM21 particles in SCGM media (CellGenix GmbH, Freiburg im Breisgau, Germany) supplemented with 10% FBS, 2 mM GlutaMAX. .. CSTX-002 cells (K562 cell line expressing 41BBL and membrane-bound IL-21) used for preparation of PM21 particles were provided by Kiadis Pharma, a Sanofi company.

    Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy
    Article Snippet: Cells that were being stored for future irradiations were suspended at 10 7 cells/mL in 10% DMSO in FBS and kept in a Mr. Frosty freezing container (ThermoFisher Cat# 5100-0001) at −80°C for a few days for gradual cooling before being transferred to −150°C for long term storage. .. CD3+ lymphocytes were isolated from fixed blood samples - collected via finger prick for healthy donors and blood draw for cancer patients - using the EasySep Human Whole Blood CD3 Positive Selection Kit (StemCell Tech Cat# 18081). .. Briefly, fixed blood was incubated with 100 μL of red blood cell lysis buffer (StemCell Tech Cat# 20110) for 15 min at room temperature in the 96-well plate.

    Article Title: Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo .
    Article Snippet: .. Briefly, whole PBMCs or T-celldepleted PBMCs (EasySep CD3 Positive Selection Kit; StemCell Technologies, Vancouver, Canada) were cultured for 13–14 days with 100 U/mL IL-2 (PeproTech, Cranbury, NJ, USA) and 200-μg/mL PM21 particles in SCGM media (CellGenix GmbH, Freiburg im Breisgau, Germany) supplemented with 10% FBS, 2 mM GlutaMAX. .. CSTX-002 cells (K562 cell line expressing 41BBL and membrane-bound IL-21) used for preparation of PM21 particles were provided by Kiadis Pharma, a Sanofi company.

    Cell Culture:

    Article Title: Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo
    Article Snippet: .. Briefly, whole PBMCs or T-cell-depleted PBMCs (EasySep CD3 Positive Selection Kit; StemCell Technologies, Vancouver, Canada) were cultured for 13–14 days with 100 U/mL IL-2 (PeproTech, Cranbury, NJ, USA) and 200-μg/mL PM21 particles in SCGM media (CellGenix GmbH, Freiburg im Breisgau, Germany) supplemented with 10% FBS, 2 mM GlutaMAX. .. CSTX-002 cells (K562 cell line expressing 41BBL and membrane-bound IL-21) used for preparation of PM21 particles were provided by Kiadis Pharma, a Sanofi company.

    Article Title: Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo .
    Article Snippet: .. Briefly, whole PBMCs or T-celldepleted PBMCs (EasySep CD3 Positive Selection Kit; StemCell Technologies, Vancouver, Canada) were cultured for 13–14 days with 100 U/mL IL-2 (PeproTech, Cranbury, NJ, USA) and 200-μg/mL PM21 particles in SCGM media (CellGenix GmbH, Freiburg im Breisgau, Germany) supplemented with 10% FBS, 2 mM GlutaMAX. .. CSTX-002 cells (K562 cell line expressing 41BBL and membrane-bound IL-21) used for preparation of PM21 particles were provided by Kiadis Pharma, a Sanofi company.

    Isolation:

    Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy
    Article Snippet: Cells that were being stored for future irradiations were suspended at 10 7 cells/mL in 10% DMSO in FBS and kept in a Mr. Frosty freezing container (ThermoFisher Cat# 5100-0001) at −80°C for a few days for gradual cooling before being transferred to −150°C for long term storage. .. CD3+ lymphocytes were isolated from fixed blood samples - collected via finger prick for healthy donors and blood draw for cancer patients - using the EasySep Human Whole Blood CD3 Positive Selection Kit (StemCell Tech Cat# 18081). .. Briefly, fixed blood was incubated with 100 μL of red blood cell lysis buffer (StemCell Tech Cat# 20110) for 15 min at room temperature in the 96-well plate.



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    Impact of whole blood and selected <t>CD3</t> + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%
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    (A) Baseline number of foci per nucleus as a function of age in <t>CD3</t> + lymphocytes extracted from finger prick collection in 339 healthy donors (***p < 0.0001, deviation from zero of the slope of linear regression). Four donors in this cohort were considered healthy but were in remission from cancer at the time of the finger prick (shown as red dots). See also . (B) Distribution of spontaneous foci per nucleus after bead-based extraction of CD3 + lymphocytes from finger prick samples (finger prick), Ficoll-based extraction of PBMCs from buffy coat samples (PBMC baseline), and a further freeze-thaw cycle and plating in the cell culture media (PBMC 0 Gy). ***p < 0.0001, one-way ANOVA, Tukey’s post hoc test for multiple comparisons among all three groups: 1–2, 2–3, and 1–3. Boxplots show median ± quartiles ± min/max values.
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    easysep hla whole blood cd3 positive selection kit  (STEMCELL Technologies Inc)
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    (A) Baseline number of foci per nucleus as a function of age in <t>CD3</t> + lymphocytes extracted from finger prick collection in 339 healthy donors (***p < 0.0001, deviation from zero of the slope of linear regression). Four donors in this cohort were considered healthy but were in remission from cancer at the time of the finger prick (shown as red dots). See also . (B) Distribution of spontaneous foci per nucleus after bead-based extraction of CD3 + lymphocytes from finger prick samples (finger prick), Ficoll-based extraction of PBMCs from buffy coat samples (PBMC baseline), and a further freeze-thaw cycle and plating in the cell culture media (PBMC 0 Gy). ***p < 0.0001, one-way ANOVA, Tukey’s post hoc test for multiple comparisons among all three groups: 1–2, 2–3, and 1–3. Boxplots show median ± quartiles ± min/max values.
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    Image Search Results


    Impact of whole blood and selected CD3 + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%

    Journal: Hla

    Article Title: Utility of assessing CD3 + cell chimerism within the first months after allogeneic hematopoietic stem‐cell transplantation for acute myeloid leukemia

    doi: 10.1111/tan.14557

    Figure Lengend Snippet: Impact of whole blood and selected CD3 + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%

    Article Snippet: CD3 + cells were isolated from 7 ml peripheral blood samples using the EasySep® Human Whole Blood CD3 Positive Selection Kit (STEMCELL Technologies, Vancouver, BC, Canada).

    Techniques:

    (A) Baseline number of foci per nucleus as a function of age in CD3 + lymphocytes extracted from finger prick collection in 339 healthy donors (***p < 0.0001, deviation from zero of the slope of linear regression). Four donors in this cohort were considered healthy but were in remission from cancer at the time of the finger prick (shown as red dots). See also . (B) Distribution of spontaneous foci per nucleus after bead-based extraction of CD3 + lymphocytes from finger prick samples (finger prick), Ficoll-based extraction of PBMCs from buffy coat samples (PBMC baseline), and a further freeze-thaw cycle and plating in the cell culture media (PBMC 0 Gy). ***p < 0.0001, one-way ANOVA, Tukey’s post hoc test for multiple comparisons among all three groups: 1–2, 2–3, and 1–3. Boxplots show median ± quartiles ± min/max values.

    Journal: Cell reports

    Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy

    doi: 10.1016/j.celrep.2020.108434

    Figure Lengend Snippet: (A) Baseline number of foci per nucleus as a function of age in CD3 + lymphocytes extracted from finger prick collection in 339 healthy donors (***p < 0.0001, deviation from zero of the slope of linear regression). Four donors in this cohort were considered healthy but were in remission from cancer at the time of the finger prick (shown as red dots). See also . (B) Distribution of spontaneous foci per nucleus after bead-based extraction of CD3 + lymphocytes from finger prick samples (finger prick), Ficoll-based extraction of PBMCs from buffy coat samples (PBMC baseline), and a further freeze-thaw cycle and plating in the cell culture media (PBMC 0 Gy). ***p < 0.0001, one-way ANOVA, Tukey’s post hoc test for multiple comparisons among all three groups: 1–2, 2–3, and 1–3. Boxplots show median ± quartiles ± min/max values.

    Article Snippet: CD3+ lymphocytes were isolated from fixed blood samples - collected via finger prick for healthy donors and blood draw for cancer patients - using the EasySep Human Whole Blood CD3 Positive Selection Kit (StemCell Tech Cat# 18081).

    Techniques: Extraction, Cell Culture

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy

    doi: 10.1016/j.celrep.2020.108434

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: CD3+ lymphocytes were isolated from fixed blood samples - collected via finger prick for healthy donors and blood draw for cancer patients - using the EasySep Human Whole Blood CD3 Positive Selection Kit (StemCell Tech Cat# 18081).

    Techniques: Recombinant, Red Blood Cell Lysis, Selection, Flow Cytometry, Multiplex Assay, Software